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. 2010 Nov;12(11):946–956. doi: 10.1593/neo.10790

Figure 1.

Figure 1

B-Raf kinase activation induces HPR1 expression. (A) Induction of HPR1 gene expression. HEK293 cells were transiently transfected with the pEF6 empty vector or the vector encoding wild-type BRAF, BRAFV600E, or HRASG12V. Total RNA was extracted and analyzed for HPR1 mRNA expression by RT-PCR. (B) Induction of HPR1 promoter-driven luciferase reporter gene expression. HEK293 cells were cotransfectedwith the empty vector or the vector encoding wild-type BRAF, BRAFV600E, or HRASG12V plus control luciferase reporter or the luciferase reporter gene driven by a 0.7- or 3.5-kb HPR1 promoter. pCMV/SPORT, which encodes a β-galactosidase gene, was included as an internal control. After incubating for 48 hours, the cells were harvested and analyzed for luciferase activity. The relative luciferase activity was calculated. One representative example in triplicate from three experiments with similar results is presented.