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Decreased HPR1 promoter activity in BRAF siRNA-transfected KAT-10 cellls. Control or BRAF siRNA-transfected KAT-10 cells were transiently transfected with the luciferase reporter gene expression driven by a 0.3-, 0.7-, or 3.5-kb HPR1 promoter. pGL3/Basic luciferase reporter construct was included as a negative control. After incubating for 48 hours, the cells were harvested and analyzed for luciferase activity.
