Figure 5.

Evidence that the GABP binding site in the HPR1 promoter is critical for BRAF-mediated HPR1 gene regulation. (A) Schematic illustration of a panel of the HPR1 promoter-driven luciferase reporter constructs. ERE sites marked with a cross sign denote those with altered nucleotides can no longer be bound by Ets transcription factors. (B) Control or BRAF siRNA-transfected KAT-10 cells were transiently transfected with the luciferase reporter constructs as shown in (A) or with 4xGABP binding site-driven luciferase reporter construct (C). After incubating for 48 hours, the cells were harvested and analyzed for luciferase activity.