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. 2010 Nov;12(11):946–956. doi: 10.1593/neo.10790

Figure 6.

Figure 6

Figure 6

Down-regulation of GABPβ in BRAF-suppressed KAT-10 cells. (A) IF staining of GABPα and β. Control or BRAF siRNA-transfected KAT-10 cells were stained with an anti-GABPα and β rabbit IgG, followed by staining with fluorescein-conjugated goat antirabbit IgG. A normal rabbit antiserum was included as a negative control. No nonspecific signal was presented (photograph not shown). (B) Western blot analyses of the signaling molecules and transcription factors involved in regulating HPR1 gene expression. Cell lysates from control or BRAF siRNA-transfected KAT-10 cells were analyzed for the expression of several transcription factors and signaling molecules by their specific antibodies. (C) Real-time RT-PCR analyses of GABPα and β expression. Total RNA was extracted fromKAT-10 cells transfected with BRAF or control siRNA expression vector and analyzed for GABPα and β by real-time RT-PCR. One of two experiments in triplicate with similar results is shown.