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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cancer Res. 2010 Oct 5;70(20):8045–8054. doi: 10.1158/0008-5472.CAN-10-2352

Figure 1.

Figure 1

Hypoxia decreases homologous recombination independent of PARP activity. (A) RAD51 protein expression is decreased in RKO cells following 16 h × 0.02% O2 and remains suppressed for at least after 8 h of reoxygenation with or without ABT-888. (B) RAD51 protein expression is decreased in H1299, DU145, RKO, HCT116, and MCF7 cancer cells as well as HIF-1α-isogenic MEFs following 72 h × 0.2% O2 with or without ABT-888. (C) Functional HR (as assessed by the DR-GFP assay) is decreased following 72 h × 0.2% O2 but is unaffected by ABT-888 in H1299 cells. Representative dot plots are shown. Columns, mean of 3 experiments; bars, SE; *, P<0.05.