Figure 4.

Localization of PDYN and Dynorphins, and Analysis of the Purkinje Cell-Specific Excitatory Amino Acid Transporter, in SCA23 Cerebellum

(A) Immunohistochemistry of cerebellum from the PDYN p.R138S subject and a control individual without neurological disease. Immunoperoxidase labeling with anti-Dyn A, anti-Dyn B, or anti-PDYN antibodies was visualized by 3-Amino-9-ethylcarbazole as the chromogen and was counterstained with hematoxylin.

(B) Immunoblot analysis of excitatory amino acid transporter (EAAT4) and calbindin, both Purkinje cell markers. High molecular weight species were detected in the SCA23 cerebellum (^∗).

(C) Immunoperoxidase labeling of EAAT4 in SCA23 and control cerebellum.

Scale bars represent 30 μm (A and C).