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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: Transplantation. 1999 Sep 27;68(6):747–757. doi: 10.1097/00007890-199909270-00006

Figure 2.

Figure 2

Cytotoxic activity of freshly isolated normal (C3H) heart nonparenchymal cells, heart graft-infiltrating cells (GIC), and recipient (C3H) spleen cells against target cells (EL4) expressing donor (B10) alloantigen (H-2b). A 4-hr 51Cr-release assay was used to determine cytotoxicity 8 or 14 days after organ transplantation. Treatment of heart graft recipients with immature DC + anti-CD40L mAb 7 days before heart transplantation (HTX) inhibited the anti-donor CTL response, both within the graft, and systemically. By day 14, these responses were profoundly suppressed. Cells were pooled from groups of eight normal or three transplanted animals. Results, calculated as described in Materials and Methods, are means:± 1 SD, and are representative of two separate experiments.