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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: Transplantation. 1999 Sep 27;68(6):747–757. doi: 10.1097/00007890-199909270-00006

Figure 7.

Figure 7

Up-regulation of costimulatory molecule expression on immature DC after exposure to allogeneic T cells is not inhibited in the presence of anti-CD40L mAb. Immature B10 (H-2b) DC were cocultured with purified allogeneic naive C3H (H-2k) T cells (DC: T cells = 1:2) for 18 hr (CD40, CD86) or 36 hr (CD80) in the absence or presence of anti-CD40L mAb (10 μg/ml). Expression of CD40, 80, and 86 was then determined on gated cells by flow cytometric analysis (T cells were gated out by staining with anti-CD3 mAb). Open profiles denote Ig isotype controls.