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. Author manuscript; available in PMC: 2010 Nov 12.
Published in final edited form as: Horm Metab Res. 1984 May;16(5):237–242. doi: 10.1055/s-2007-1014755

Table 3.

Stimulation of DNA synthesis in Novikoff hepatoma cells by HSA from various sources

Source of Extract μg protein of HSA
50 100 200
[3H] thymidine incorporation (cpm/106 cells)
None 640 ± 110
Sham operated rats 683 ± 84 627 ± 27 397 ± 62
Weanling rats 966 ± 151 1222 ± 185 857 ± 45
70% hepatectomy 1402 ± 210 1856 ± 150 1229 ± 160
T3 injected rats 774 ± 53 1030 ± 120 1325 ± 82
EtOH fractions from weanling rats V 928 ± 105 1139 ± 95 1990 ± 270
VI 986 ± 75 1594 ± 110 2048 ± 86

The assay has been described in “Materials and Methods”. Cell density was between 1.5 and 2 × 106 at termination of experiment. Cells were exposed to 0.5 μCi [3H] thymidine per dish for 2 hours, 20 hours after addition of HSA in a total volume of 4 ml medium containing 0.1% calf serum. Numbers are the averages of 3 or more individual dishes ± S.D.