. Author manuscript; available in PMC: 2010 Nov 12.

Published in final edited form as: Horm Metab Res. 1984 May;16(5):237–242. doi: 10.1055/s-2007-1014755

Table 4.

Stimulation of DNA synthesis in primary cultures of hepatocytes by liver extracts

Source of HSA	Protein Added (μg)	[³H] thymidine Incorporation CPM/10⁶ cells	% Increase
None	0	1630 ± 95	–
Sham hepatectomy	50	1862 ± 167	14
	100	1741 ± 220	7
Weanling rat	50	3717 ± 960	128
	100	2633 ± 325	62
Adult 70% hepatectomy	50	1891 ± 41	16
	100	2266 ± 196	39
T₃ injected rat	50	2103 ± 253	29
	100	2543 ± 211	56

Hepatocytes were plated at a cell density of 2 × 10⁶ cells per 60 mm dish in 4 ml medium. Medium was changed at 4 and 24 hours. Additions were made as indicated at 24 hours and cells were pulsed for 16 hours with 2 μCi [³H] thymidine per dish. Label was added 8 hours after HSA additions. Numbers are the averages of 3 determinations ± S.D.