Figure 6.

Expression of cathepsins in normal basal ganglia (A, C, E, G, H) and ischemic basal ganglia at 2-hour MCAO (B, D, F, I to L) in frozen (A to F) and paraffin sections (G to L). dUTP uptake is seen in ischemic issue (B) but not normal tissue (A). Cathepsin B (C, D, G, I, K) and cathepsin L (E, F, H, J, L) expression are demonstrated. A to F, Both cathepsin B and L immunoreactivity are seen in the border of the ischemic core region but not in normal tissue. G to L, Cathepsin B is observed on both glial cells (I) and on nearby microvessels (K) in the ischemic core/ischemic peripheral border, while cathepsin L is observed on neurons (J) and adjacent microvessels (L) in the same region. Bars=2 mm (A to F), 10 µm (G, H), 5 µm (I, J), and 2.5 µm (K, L).