Loss of dystroglycan expression in Nestin-Cre/DG-null mouse brain. A, Western blot analysis of multiple brain regions from wild-type littermate control (C) and Nestin-Cre/DG-null (N) adult brain. B, C, Immunofluorescence detection of β-dystroglycan (green) in the cerebrum of wild-type littermate control (B) and Nestin-Cre/DG-null (C) mice. D, E, Immunofluorescence detection of laminin (green) in littermate control (D) and Nestin-Cre/DG-null (E) mice. Scale bars, 200 μm.