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. 2010 Aug 4;299(5):C994–C1006. doi: 10.1152/ajpcell.00544.2009

Fig. 1.

Fig. 1.

Expression of telomerase and osteoblastic markers in primary calvarial osteoblasts and human telomerase reverse transcriptase (hTERT)-immortalized cell lines. A: telomerase expression in hTERT-immortalized mouse wild-type (MOB) and connexin43 (Cx43)-null (43KO-MOB) osteoblastic cell lines detected by Western blotting. Equal amounts of protein from HeLa, MOBs, 43KO-MOBs, MC3T3-E1, primary mouse wild-type osteoblasts (PMOB), and Cx43-null osteoblasts (43KO-PMOB) were used. HeLa cells were used as positive control. B: RT-PCR analysis of osteoblastic markers (osteopontin, OSF-2, PTH/PTHrP-R, osteocalcin, and type I collagen), two transcription factors associated with bone formation and mineralization [runt-related transcription factor 2 (Runx2) and osterix (Osx)] and β-actin (loading control) in MOBs and 43KO-MOBs. The expression of the osteoblastic markers and bone formation and mineralization associated transcription factors from 10-day culture of the hTERT-immortalized cells were compared with those of wild-type calvarial tissue, primary wild-type and Cx43-null osteoblasts, MC3T3E-1, and HeLa (negative control).