Fig. 9.

Effect of Cx43 deletion on expression levels of Runx2 and osterix. Wild-type MOB and 43KO-MOB mouse osteoblastic cell lines (seeded at 1.5 × 10³ cells/cm²) were plated in 35-mm dishes and cultured in α-MEM + 10% FBS for 6 and 9 days. Quantitative real-time PCR analysis of Runx2 (A) and osterix (B) mRNA expression in 43KO-MOB-C relative to MOB-C at 6 and 9 days after platting. The ΔΔCT method was used for data analysis where the value obtained for each gene of interest is first normalized to the reference gene (β-actin) and then to that obtained for MOB-C (indicated by horizontal lines). All data are presented as means ± SE, N = 3; *P < 0.05, **P < 0.005, ***P < 0.0005. P values were obtained using t-tests.