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ERK activation in response to α1-receptor stimulation requires ATP. HEK-293 cells were treated with apyrase (apy; 20 U/ml), a scavenger of extracellular ATP. After 10 min, the cells were stimulated with the α1-receptor agonist phenylephrine (10 μM), and ERK activation was determined as described above. Cells stimulated with ATP (20 μM) and ATPγS (20 μM) were included as positive controls.
