Fig. 4.

SV2A-R231Q, W300A, and W666A are trafficked to synapses. Autaptic hippocampal neurons from SV2A/B double KO mice were infected with Lenti virions encoding the indicated SV2A-EGFP fusion protein. Cultures were fixed and processed for immunolabeling with monoclonal anti-synaptophysin and polyclonal anti-SV2A antibodies. Antibody binding was visualized with goat-anti-rabbit Alexa-Fluor 488 (green) and goat-anti-mouse Alexa-Fluor 568 (red). Shown are images collected with a Nikon upright microscope. SV2A-EGFP, SVA-R231Q, W300A, and W666A trafficked properly to the synapses and colocalized with synaptophysin.