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Kv1 subunit expression in cDNA from passage 6 synoviocytes. A: products of appropriate size were obtained for Kv1.1, Kv1.4, Kv1.5, and Kv1.6 following 35 cycles of PCR amplification (n = 3). B: as a positive control, cDNA from rabbit brain was examined using the same primers. All eight Kv1 subtypes were found in rabbit brain. C and D: immunostaining with a mouse monoclonal antibody against Kv1.1 (C) and the control experiment with only secondary antibody present (D).
