Fig. 4.
Cell surface expression of wild-type and mutant BCRP. Expression of wild-type and mutant BCRP on cell surface of stably transfected HEK-293 cells was detected using the monoclonal antibody 5D3, as described. Representative flow cytometry histograms showing cell surface expression of wild-type and mutant BCRP are presented. The open and solid peaks represent the phycoerythrin fluorescence associated with cells treated with the IgG2b negative control and the 5D3 antibodies, respectively. No surface expression of BCRP was detected in the pcDNA vector control cells. The experiments were repeated three times, and similar results were obtained. FL2-H, histogram of the FL2 peak fluorescence emission values.