Fig. 4.

Ad increases acetyl-CoA carboxylase (ACC) phosphorylation and FA oxidation and decreases ACC activity and intracellular lipid content. A: representative immunoblot and quantification of ACC phosphorylation (Ser⁷⁹). B: quantification of ACC activity in adult cardiomyocytes. ACC activity determined by incorporation of [¹⁴C]sodium bicarbonate into malonyl-CoA in adult cardiomyocytes stimulated with or without 10 μg Ad for 1 or 2 h or with 1 mM 5-aminoimidazole-4-carboxamide-1-β-d-ribofuranoside (AICAR) for 2 h. C: quantification of palmitate oxidation in cardiomyocytes untreated (0 h) or treated with 10 μg Ad for 2 or 6 h or with 1 mM AICAR for 2 h. Representative confocal images (D) and Quantification of intracellular lipid content (E) assayed using Oil red O staining in cardiomyocytes treated with 10 μg Ad or without (CON) for 20 h. Data are means ± SE of ≥4 independent experiments and are expressed relative to time-matched control (0 min). *P < 0.05 vs. Control.