Fig. 5.

Effects of Ad on substrate metabolism, signaling molecules, and cardiac performance in perfused working mouse hearts. Representative immunoblot and quantification of phospho-AMPK^Thr172 (A), phospho-ACC^Ser79 (B), and total AMPKα1, AMPKα2, ACC, LKB1, and APPL1 (C) in the same hearts in which metabolism was measured. Quantification of Ad's effects on palmitate oxidation (D), glucose oxidation (E), glycolysis (F), oxygen consumption (MV̇o₂; G), hydraulic work (H), and cardiac efficiency (I) in perfused hearts. All parameters were measured in hearts (n = 5) isolated from 6-wk-old mice perfused with 4 μg/ml Ad or without (CON) for 60 min. Data are means ± SE from, and are expressed relative to, hearts perfused without Ad for 60 min (CON). *P < 0.05 vs. CON.