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Subcellular semiquantitative analyses of key β-cell proteins involved in β-cell function and proliferation. Semiquantitative confocal analysis of the β-cell nuclear signal showed no differences in the key factors pancreatic/duodenal homeobox-1 (Pdx1; A) and MafA (B) between the CVX and control groups or in the cell cycle inhibitor p27 (C). D: a trend in the reduction of nuclear cyclin D2, the principal cyclin D isoform in β-cells, was observed.
