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Proteasome activity was not changed in mdx animals following 3 mo of a BBIC (+1% BBIC, —) diet compared with control diet (mdx, —) (n = 3–8 per group). Also, addition of exogenous BBIC or purified BBI (+BBI) to the assay did not affect proteasome activity, suggesting this inhibitor does not directly inhibit proteasome activity.
