Fig. 4.

Time course effects of different doses of SKF-96365 (A) and NiCl₂ (B) on [Ca²⁺]_i change (Δ[Ca²⁺]_i) before and after restoration of extracellular [Ca²⁺] to 2.5 mM in distal PVSMC perfused with Ca²⁺-free KRB solution containing 10 μM CPA, 1 mM EGTA, and 5 μM nifedipine. Average peak change in Δ[Ca²⁺]_i before (C, between 0 and 15 min) and after (D, between 15 and 30 min) restoration of extracellular [Ca²⁺] in cells exposed to SKF-96365 (1, 10, and 50 μM; C), NiCl₂ (50, 200, and 500 μM; D), or vehicle control. *Significant difference from respective control (P < 0.05). **P < 0.05 vs. value at 1 μM SKF-96365 or 50 μM NiCl₂. ***P < 0.05 vs. value at 10 μM SKF-96365 or 200 μM NiCl₂.