Fig. 5.

A: effect of 50 μM SKF-96365 and 500 μM NiCl₂ on time course of fura-2 fluorescence at 360 nm normalized to values at time 0 before and after administration of MnCl₂ (200 μM) to distal PVSMC perfused with Ca²⁺-free KRB solution containing 10 μM CPA, 1 mM EGTA, and 5 μM nifedipine. B: average change in fura-2 fluorescence at 360 nm in cells exposed to SKF-96365 (n = 5 experiments in 120 cells), NiCl₂ (n = 5 experiments in 118 cells), or vehicle control (n = 5 experiments in 117 cells) at 10 min after administration of MnCl₂. *Significant difference from control (P < 0.0001).