Table 1.

Real-time PCR primers for rat TRPCs, TRPVs, and β-actin

Gene	Accession No.	Primer Sequence, Left/Right	Product Size, bp	Location in Sequence	Positive Control
TRPC1	NM_053558	5′-AGCCTCTTGACAAACGAGGA-3′	146	797–942	Brain
		5′-ACCTGACATCTGTCCGAACC-3′
TRPC2	NM_022638	5′-TTGCTGGGAGAGTCTCTGGT-3′	139	1235–1393	Brain
		5′-CCTCGATCCACACCTCCTTA-3′
TRPC3	NM_021771	5′-GAGATCTGGAATCGGTGGAA-3′	127	608–754	Brain
		5′-AAAAGCTGCTGTTGGCAGTT-3′
TRPC4	NM_053434	5′-GACACGGAGTTCCAGAGAGC-3′	142	771–912	Brain
		5′-GTTGGGCTGAGCAACAAACT-3′
TRPC5	NM_080898	5′-CCGCAAGGAGGTGGTAGG-3′	121	643–784	Brain
		5′-TGTGATGTCTGGTGTGAACTC-3′
TRPC6	NM_053559	5′-TACTGGTGTGCTCCTTGCAG-3′	141	1267–1407	Brain
		5′-GAGCTTGGTGCCTTCAAATC-3′
TRPC7	XM_225159	5′-AACGAGACCTTCACAGACTAC-3′	114	1270–1383	Brain
		5′-GCATTCAGACCAGATCATTCC-3′
TRPV1	NM_031982	5′-GCGAGTTCAAAGACCCAGAG-3′	79	517–595	Brain
		5′-GTGTCATTCTGCCCATTGTG-3′
TRPV2	NM_017207	5′-GCTGGCTGAACCTGCTTTAC-3′	138	1865–2002	Brain
		5′-CTACAGCAAAGCCGAAAAGG-3′
TRPV3	NM_001025757	5′-AGTGCCTCTCTGGCAACTGT-3′	122	322–443	Small intestine
		5′-CTGCCTCTGTTCTTCCTTGG-3′
TRPV4	NM_023970	5′-AGCAACCTGGAGACTGTGCT-3′	143	1171–1313	Brain
		5′-TTGAACTTGCGAGACAGGTG-3′
TRPV5	NM_053787	5′-GTCCTTTCTGGAGCTTGTGC-3′	75	1014–1088	Kidney
		5′-TCCTTCACAGGGGTTTGTTC-3′
TRPV6	NM_053686	5′-CTGGTTTTTGAGCCCATGAC-3′	93	605–697	Brain
		5′-AGCACGGACCAAGTTCACAT-3′
β-actin	NM_031144	5′-ACGGTCAGGTCATCACTATC-3′	90	812–901	Brain
	5′-TGCCACAGGATTCCATACC-3′