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. 2010 Aug 18;299(5):F1065–F1077. doi: 10.1152/ajprenal.00277.2010

Fig. 5.

Fig. 5.

Effect of IC-Rhbg-KO on renal ammonia excretion in response to metabolic acidosis. A: urinary ammonia excretion in IC-Rhbg-KO and C mice. Mice were placed on control diet for 1 day and then changed to HCl diet. On days 2, 3, and 4 of acid loading, IC-Rhbg-KO mice excreted significantly less urinary ammonia than did C mice. B: urine titratable acid excretion in IC-Rhbg-KO and C mice. There was no significant difference in urine titratable acid excretion either before acid loading or at any time point during the HCl acid-loading protocol. C: urine pH during the same time period. Urine pH decreased significantly with induction of metabolic acidosis in both IC-Rhbg-KO and C mice, but it did not differ significantly between IC-Rhbg-KO and C mice either on control diet or any day after acid loading (*P < 0.05 vs. pH before acid loading). D: food intake in IC-Rhbg-KO and C mice. HCl acid loading was accomplished by adding 0.4 M HCl to powdered standard rodent chow. Food intake, and therefore the acid load, did not differ significantly between C and IC-Rhbg-KO mice either before or during acid loading. E: urinary Na+ excretion in C and IC-Rhbg-KO mice. There was no significant difference in urinary Na+ excretion between C and IC-Rhbg-KO mice either under basal conditions or in response to acid loading. F: urinary K+ excretion in C and IC-Rhbg-KO mice. There was no significant difference in urinary K+ excretion between C and IC-Rhbg-KO mice either under basal conditions or in response to acid loading. N = 18 for both C and IC-Rhbg-KO before acid loading and on days 1-3 of acid loading and N = 10 on days 4 and 5 of acid loading in all panels. *P < 0.05 vs. C mice.