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Effect of increasing and decreasing luminal flow on nitric oxide (NO) production by isolated thick ascending limbs (TALs; *P < 0.02 compared with 0 nl/min, period 1; **P < 0.02 compared with 20 nl/min, period 1; #P < 0.04 compared with 0 nl/min, period 2; n = 7). There was no difference between flow-stimulated NO production during periods 1 and 2.
