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Nucleofection does not alter cilia length. MDCK cells treated as indicated were plated on filters for 4 days and then fixed and processed for indirect immunofluorescence using monoclonal anti-acetylated β-tubulin antibody. A: representative confocal image and inset for each condition. Bar = 10 μm. B: ImageJ software was used to measure the distribution of cilia lengths from 50 randomly acquired images for each condition.
