Fig. 6.

TM11 mutant rNCC and the single mutant S575C-rNCC exhibited higher activity than wild-type NCC or I574C-rNCC. Oocytes were injected with the same amount of cRNA of each clone, as explained in the text. A: 3 days later the ²²Na⁺ uptake was assessed in the absence (open bars) or presence (filled bars) of metolazone. Data presented are means ± SE of the uptake observed for each oocyte. Similar observations were done in at least 5 experiments. *P < 0.05 vs. control wild-type rNCC. B: proteins were extracted for a Western blot analysis using anti-FLAG or anti-actin antibody. A representative Western blot is shown in B. C: densitometric analysis of bands observed in the Western blot. OD, optical density.