Fig. 3.
Ang-(1–7) stimulates NO through nuclear Ang-(1–7) receptors. A: purified nuclei were stimulated with Ang-(1–7) (1 nM) in the presence of the AT1 antagonist LOS (1 μM), the AT2 antagonist PD (1 μM), DALA (1 μM), or the NOS inhibitor l-NAME (1 mM). Data are expressed as means ± SE; n = 7; *P < 0.05 vs. Ang-(1–7). B: immunodetection of Ang-(1–7)/Mas receptor. Purified nuclear extracts were probed with antibodies against the Mas receptor (1:200) or the nuclear membrane marker lamin A/C (1:500). Lanes 1–3 represent nuclei prepared from 3 different preparations; M is the lane for molecular weight markers.