Table 4.
Analysis of colocalization between intermediate filament and hemidesmosomal proteins in exstrophic and normal bladder epithelial cells
| Exstrophy Specimen 1 |
Exstrophy Specimen 2 |
Normal Bladder |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| IA6 |
L5 |
IA6 |
L5 |
IA6 |
L5 |
|||||||
| Rp | M | Rp | M | Rp | M | Rp | M | Rp | M | Rp | M | |
| K7 | 0.01 | 0.02 | 0.04 | 0.06 | 0.12 | 0.15 | 0.13 | 0.05 | 0.01 | 0.03 | 0.08 | 0.09 |
| K13 | 0.00 | 0.01 | 0.06 | 0.06 | 0.02 | 0.01 | 0.02 | 0.02 | 0.00 | 0.05 | 0.03 | 0.04 |
| K14 | 0.02 | 0.03 | 0.08 | 0.09 | 0.00 | 0.00 | 0.05 | 0.06 | 0.01 | 0.02 | 0.10 | 0.11 |
Cells were grown on glass coverslips as described in materials and methods. Shown is immunoreactivity with anti-integrin α6 (IA6) IgG, anti-laminin-5 (L5) IgG, and anti-keratin (K) IgG. Rp, Pearsons's correlation coefficient, scale −1 to 1; M, Manders overlap coefficient, scale 0 to 1. For both coefficients, score of 1 represents perfect colocalization.