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Effect of in vivo acidosis on Na+/H+ exchanger activity in mouse proximal convoluted tubules perfused in vitro. Proximal convoluted tubules were perfused in vitro from 8-day-old mice that were gavaged with vehicle or ammonium chloride for 4 days. Na+/H+ activity was assessed by measuring the rate of change in cell pHi after luminal sodium removal. Na+/H+ activity was significantly faster in the neonatal mice that were acidemic compared with control. No. of tubules is in parentheses.
