Fig. 1.

Semiquantitative immunoblots probed for the Na⁺/H⁺ exchanger type 3 (NHE3), bumetanide-sensitive Na⁺-K⁺-2Cl⁻ cotransporter 2 (NKCC2), thiazide-sensitive cotransporter (NCC), and the three subunits of the epithelial sodium channel (ENaC) (α, β, and γ; 70-kDa and 85-kDa forms). A: immunoblots compare whole kidney homogenates from six virgin and four mid-pregnant rats (days 11–13 of pregnancy). B: band densities were normalized to virgin controls with virgin rats set at 100% and summarized as bar graphs. An unpaired t-test was performed. Values are expressed as means ± SE; *P < 0.05.