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Semiquantitative immunoblots probed for the NHE3, bumetanide-sensitive NKCC2, NCC, and the three subunits of ENaC (α, β, and γ: 70-kDa and 85-kDa forms). A: immunoblots compare whole kidney homogenates from six virgin and seven late-pregnant rats (days 18–20 of pregnancy). B: band densities were normalized to virgin controls with virgin rats set at 100% and summarized as bar graphs. An unpaired t-test was performed. Values are expressed as means ± SE; *P < 0.05.
