Table 2. Species and number of differential diagnostic and background flora strains screened across BurkDiff to validate the assay's specificity.
| Species | No. of strains | Species | No. of strains |
| Abiotrophia/Granulicatella grp | 1 | Neisseria gonorrhoeae | 4 |
| Achromobacter xylosoxidans | 1 | Neisseria meningitidis | 3 |
| Acinetobacter baumanni | 7 | Pasteurella multocida | 1 |
| Bacillus anthracis | 1 | Propionibacterium sp. | 1 |
| Bacillus cereus | 1 | Providencia stuartii | 1 |
| Bacillus sp. | 2 | Pseudomonas aeruginosa | 7 |
| Bacteroides fragilis | 1 | Rhizopus oryzae | 1 |
| Bacteroides uniformis | 1 | Rothia mucilaginosa | 1 |
| Bordetella bronchiseptica | 1 | Salmonella enterica | 1 |
| Brucella abortus | 1 | Shigella dysenteriae | 1 |
| Brucella suis | 1 | Staphylococcus arlettae | 1 |
| Candida albicans | 5 | Staphylococcus aureus | 55 |
| Candida glabrata | 2 | Staphylococcus capitis | 1 |
| Candida parapsilosis | 3 | Staphylococcus cohnii | 1 |
| Candida tropicalis | 1 | Staphylococcus epidermidis | 8 |
| Chryseobacterium indologenes | 1 | Staphylococcus equorum | 1 |
| Coagulase negative Staphylococcus | 16 | Staphylococcus gallinarum | 1 |
| Coccidioides immitis | 1 | Staphylococcus haemolyticus | 3 |
| Coccidioides posadasii | 2 | Staphylococcus hominis | 1 |
| Corynebacterium diphtheriae | 1 | Staphylococcus kloosii | 1 |
| Corynebacterium jeikeium | 1 | Staphylococcus lugdunensis | 1 |
| Coxiella burnetii | 2 | Staphylococcus saprophyticus | 2 |
| Enterobacter aerogenes | 2 | Staphylococcus xylosus | 3 |
| Enterobacter cloacae | 10 | Stenotrophomonas maltophilia | 1 |
| Enterococcus faecalis | 9 | Streptococcus agalactiae | 9 |
| Enterococcus faecium | 6 | Streptococcus anginosus | 2 |
| Escherichia coli | 11 | Streptococcus equi | 1 |
| Francisella tularensis | 2 | Streptococcus gordonii | 1 |
| Haemophilus influenzae | 4 | Streptococcus mitis | 2 |
| Haemophilus parainfluenzae | 2 | Streptococcus mutans | 1 |
| Human gDNA | 2 | Streptococcus oralis | 1 |
| Klebsiella oxytoca | 1 | Streptococcus pneumoniae | 56 |
| Klebsiella pneumoniae | 8 | Streptococcus pyogenes | 13 |
| Lactococcus lactis | 1 | Streptococcus salivarius | 2 |
| Legionella pneumophila | 1 | Streptococcus thermophilus | 1 |
| Listeria monocytogenes | 1 | Streptococcus uberis | 1 |
| Micrococcus sp. | 1 | Streptococcus viridans grp | 8 |
| Moraxella catarrhalis | 7 | Vancomycin Resistant Enterococcus | 4 |
| Mycobacterium avium | 1 | Yersinia pestis | 1 |
| Mycoplasma pneumoniae | 1 | Yersinia pseudotuberculosis | 1 |
| Total | 328 |
Out of the 328 strains from approximately 80 species, none amplified.