Table 3. S. oneidensis MR-1 peptide fragmentation patterns where mapped to theoretical spectra from organisms representing near, mid, and distant phylogenetic neighbors.
Unique Peptides Identified | |||||
Locus | Gene | S. oneidensis MR-1 | S. putrefaciens CN32 | S. denitrificans OS217 | S. Typhimurium |
Glycolysis/Entner-Doudoroff Pathway | |||||
SO0049 | gpmA | 5 | |||
SO0932 | pgk | 8 | 2 | ||
SO0933 | fba | 10 | 4 | 4 | |
SO1200 | tpiA | 3 | |||
SO2345 | gapA-2 | 7 | 3 | ||
SO2347 | gapA-3 | 4 | |||
SO2486 | eda | 3 | 2 | ||
SO2487 | edd | 5 | |||
SO2488 | pgl | 2 | |||
SO2489 | zwf | 4 | |||
SO2491 | pykA | 10 | 4 | ||
SO2644 | ppsA | 15 | 6 | 3 | |
SO3440 | eno | 8 | 3 | 2 | 2 |
SO3547 | pgi | 3 | |||
SO3991 | fbp | 4 | 3 | 2 | |
TCA Cycle | |||||
SO0343 | acnA | 5 | 3 | ||
SO0344 | prpC | 4 | |||
SO0432 | acnB | 30 | 10 | 3 | |
SO0770 | mdh | 10 | 7 | 5 | 2 |
SO0970 | SO0970 | 11 | 4 | ||
SO1484 | aceA | 2 | |||
SO1926 | gltA | 6 | 4 | 2 | |
SO1928 | sdhA | 5 | 4 | 2 | |
SO1929 | sdhB | ||||
SO1930 | sucA | ||||
SO1931 | sucB | 9 | |||
SO1932 | sucC | 5 | 2 | ||
SO1933 | sucD | 6 | 4 | ||
SO2222 | SO2222 | 12 | 2 | ||
SO2629 | icd | 50 | 12 | 4 | |
SO4118 | SO4118 | 4 |
Data represented here are from highly conserved central metabolic pathways, as unique peptide count – the number of peptides identified belonging to a protein with an ortholog in the S. oneidensis genome.