Table 4. Shewanella isolates were identified from the Columbia River, based on 16S rDNA sequencing.
Number of identified peptides | |||||||||||
S. oneidensis genome comparison | S. putrefaciens genome comparison | ||||||||||
MR-1 locus | CN32 locus | Gene | HRCR-1 | HRCR-2 | HRCR-4 | HRCR-5 | HRCR-1 | HRCR-2 | HRCR-4 | HRCR-5 | |
Glycolysis/Entner-Doudoroff Pathway | |||||||||||
SO0049 | CN32_0040 | gpmA | 3 | 2 | |||||||
SO0932 | CN32_0874 | pgk | 7 | 5 | 7 | 5 | 5 | 3 | 4 | 4 | |
SO0933 | CN32_0875 | fba | 6 | 6 | 5 | 6 | 4 | 5 | 4 | 5 | |
SO1200 | CN32_2838 | tpiA | 3 | 3 | 4 | 3 | 3 | 3 | |||
SO2345 | CN32_1889 | gapA-2 | 4 | 3 | 2 | 3 | 3 | 3 | 2 | 2 | |
SO2347 | CN32_1891 | gapA-3 | 6 | 3 | 5 | 3 | 3 | 2 | 3 | 4 | |
SO2486 | CN32_1869 | eda | |||||||||
SO2487 | CN32_1868 | edd | 2 | 2 | 2 | 3 | |||||
SO2488 | CN32_1867 | pgl | |||||||||
SO2489 | CN32_1866 | zwf | |||||||||
SO2491 | CN32_1864 | pykA | 3 | 8 | 4 | 6 | 5 | 3 | 5 | ||
SO2644 | CN32_2243 | ppsA | 11 | 7 | 9 | 8 | 8 | 4 | 5 | 3 | |
SO3440 | CN32_2757 | eno | 6 | 7 | 6 | 7 | 3 | 6 | 2 | 5 | |
SO3547 | CN32_1048 | pgi | 2 | 2 | |||||||
SO3991 | CN32_0676 | fbp | 5 | 3 | 5 | 4 | 4 | 3 | 3 | 4 | |
TCA Cycle | |||||||||||
SO0343 | CN32_3646 | acnA | 4 | 4 | 5 | 3 | 4 | 4 | 4 | 5 | |
SO0344 | CN32_3645 | prpC | 2 | 2 | 3 | 3 | 2 | 3 | 2 | 3 | |
SO0432 | CN32_3409 | acnB | 19 | 13 | 19 | 12 | 14 | 12 | 11 | 12 | |
SO0770 | CN32_3219 | mdh | 6 | 5 | 7 | 5 | 5 | 4 | 6 | 3 | |
SO0970 | CN32_0905 | SO0970 | 13 | 8 | 11 | 8 | 7 | 12 | 6 | 9 | |
SO1484 | CN32_1239 | aceA | 3 | 2 | 2 | 2 | 2 | 2 | |||
SO1926 | CN32_2274 | gltA | 4 | 4 | 5 | 6 | 5 | 3 | 3 | 4 | |
SO1928 | CN32_2271 | sdhA | 7 | 5 | 3 | 3 | 5 | 5 | 2 | 2 | |
SO1929 | CN32_2270 | sdhB | |||||||||
SO1930 | CN32_2269 | sucA | 6 | 4 | 9 | 4 | 2 | 4 | 4 | 4 | |
SO1931 | CN32_2268 | sucB | 4 | 4 | 6 | 4 | 4 | 3 | 3 | 4 | |
SO1932 | CN32_2267 | sucC | 3 | 2 | 4 | 3 | 2 | 2 | 2 | ||
SO1933 | CN32_2266 | sucD | 4 | 2 | 4 | 2 | 4 | 2 | 4 | ||
SO2222 | CN32_1807 | SO2222 | 7 | 6 | 5 | 5 | 5 | 8 | 6 | 6 | |
SO2629 | CN32_2230 | icd | 15 | 5 | 12 | 10 | 8 | 7 | 6 | 11 | |
SO4118 | CN32_0554 | SO4118 | 2 | 2 |
Peptides from these isolates were compared to 11 sequenced Shewanella genomes, with the result that the most peptides were identified when S. oneidensis MR-1 or S. putrefaciens CN32 was used as the reference genome. Data below shows the number of identified peptides from central metabolic pathways using either S. oneidensis MR -1 or S. putrefaciens CN32 genomes.