Table 1. Genes required for surviving Spc110 cleavage.
| Gene | Role | Synthetic Growth Defect in W303a |
| Mitosis | ||
| CTF19 | Kinetochore | |
| CIK1 b | MT motor (kinesin-14 accessory) | Yes |
| KAR3 b | MT motor (kinesin-14) | Yes |
| VIK1 b | MT motor (kinesin-14 accessory) | Yes |
| JNM1 b | MT motor (dynactin complex) | Yes |
| DYN3 b | MT motor (dynein inter. light ch.) | Yes |
| CLB2 | mitotic cyclin | |
| BFA1 | mitotic exit | |
| BUB3 | mitotic spindle checkpoint | |
| MAD1 b | mitotic spindle checkpoint | Yes |
| MAD3 | mitotic spindle checkpoint | |
| MAD2 b | mitotic spindle checkpoint | Yes |
| CTF18 | sister chromatid cohesion | |
| CTF8 | sister chromatid cohesion | |
| CHL1 | sister chromatid cohesion | |
| Protein Modification | ||
| RTS1 b | PP2A B' subunit | Yes |
| NCS2 b | Urmylation | Yes |
| PPM1 b | PP2A methyltransferase | No |
| UBC4 b | Ubiquitination (E2) | Yes |
| UBC7 | Ubiquitination (E2) | |
| Nuclear Pore | ||
| SAC3 b | nuclear pore | Yes |
| POM152 b | nuclear pore | Yes |
| NUP60 b | nuclear pore | Yes |
| Chromatin Remodeling | ||
| HTZ1 | Histone | |
| SIN3 | Histone deacetylase | |
| DOT1 b | Histone methyltransferase | No |
| EAF3 | Histone acetyltransferase | |
| VPS71 | Part of Swi/Snf remodeling | |
| mRNA Levels | ||
| LSM6 | mRNA catabolism | |
| NMD2 | mRNA catabolism | |
| SKI3 | mRNA catabolism | |
| LSM7 b | mRNA catabolism | No |
| SKI7 | mRNA catabolism | |
| UPF3 | mRNA catabolism | |
| PAT1 | mRNA catabolism | |
| PUS7 | mRNA splicing | |
| HCM1 b | transcriptional activator | Yes |
| Other | ||
| SLA1 | Endocytosis | |
| YOR052C | Unknown (zinc-finger protein) |
The top 36 candidates identified in the SGA screen are shown in this table. Deletion of each gene caused decreased growth in the presence of Spc110 cleavage with a p-value <2.17×10−3.
a
Each of these genes was deleted in the W303 genetic background and the synthetic growth defect verified.
b
Chosen for further study.