Table 2. The highest temperature that supports normal growth (°C) for haploid progeny of crosses between SPB remodeling candidate gene deletion strains and spc110 mutants.
Deletions whose strongest effect is on spc110 assembly mutant spc110-220 | |||||||||
WT | cik1Δ | jnm1Δ | ncs2Δ | ppm1Δ | ubc4Δ | pom152Δ | nup60Δ | mlp2Δ | |
SPC110 | 37 | 25 | 34 | 34 | 32 | 34 | 37 | 37 | 37 |
spc110-220 | 32 | <25 | 25 | <25 | 25 | 25 | 25 | 25 | 25 |
spc110-221 | 34 | 25 | 34 | 34 | 32 | 30 | 34 | 32 | 32 |
spc110-226 | 32 | 25 | 32 | 30 | 32 | 32 | 32 | 30 | 32 |
Results from Figures S1–S16 are compiled in this table: after mating each haploid spc110 mutant to each haploid deletion mutant, sporulating the diploids, and determining the genotypes of the resulting haploids, controls and double mutants were grown on YPD at several temperatures. The highest temperature that supports normal growth is listed for each haploid spc110 mutant, each haploid deletion mutant, and double mutant haploids. Double mutants that showed reduced growth compared to both single mutants are highlighted in bold. Normal growth was considered growth comparable to the best growth of the worst growing individual mutant.