Table 3. Mean SPB Fluorescence Comparison for Spc110::GFP and Spc42::GFP in wild type, ubc4Δ, and ubc5Δ strains.

	Spc110::GFP Fluorescence +/− SD	Spc42::GFP Fluorescence +/− SD
wild type	2470+/−1260	3760+/−2160
ubc4Δ	3660+/−2020	5020+/−3570
ubc5Δ	2090+/−1340	3870+/−1860

Asynchronously growing cells were imaged and SPB fluorescence was measured for wild type, ubc4Δ, and ubc5Δ strains. SPB fluorescence was plotted and the mean and standard deviation (SD) were determined by fitting a Gaussian curve to the distribution. Data from a representative experiment is shown. Fluorescence values are in arbitrary units.