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. 2010 Nov 12;5(11):e13970. doi: 10.1371/journal.pone.0013970

Figure 4. Lck enhances the association of RhoH with ZAP-70.

Figure 4

HEK293 cells were transfected with combinations of plasmids expressing HA-RhoH, constitutively active Lck (CA-Lck), or ZAP-70. Total lysates of transfected HEK293 cells were immunoprecipitated using anti-HA or anti-ZAP-70 antibodies. IP products (A) and total lysates (B) were analyzed by immunoblotting. Asterisks denote IgG light chains. (C) Binding activities of CA-Lck and HA-RhoH to ZAP-70 and Tyr-phosphorylation levels of HA-RhoH. Relative amounts of bound ZAP-70 or total protein levels of the Lck and HA-RhoH and Tyr-phosphorylated HA-RhoH in (A) and (B) were quantified by densitometric measurements. Binding activities were calculated by the ratio of protein amounts of CA-Lck and HA-RhoH bound in ZAP-70 IP products to total lysates. Figure shows data from one of at least three independent experiments.