Figure 2. Domains of Sox2 used for association with Sall4.

(A) Schematic diagrams of the Flag-Sox2 expression constructs used for domain mapping studies. (B) Mapping domains of Sox2 that mediate its association with Sall4. 293T cells were transiently transfected with an expression construct for Sall4 and the Sox2 constructs shown, and nuclear extracts were prepared 1 day later. Nuclear extracts (input lanes) used for co-immunoprecipitation of Sall4 are presented as western blot analyses, probing for Sall4 (top-left) or the Flag-Sox2 constructs indicated (bottom-left). M2-beads were used to co-immunoprecipitate Flag-Sox2 constructs and their associated proteins. Immunoprecipitate eluates were used in western blot analysis (right panels), and were probed for either Sall4 (top-right) or the Flag-Sox2 constructs (bottom-right). These experiments were repeated, and similar results were observed.