Figure 4. Co-immunoprecipitation of endogenous 293T cell HDAC2 by exogenous Flag-Sox2 constructs.

The indicated constructs were transiently transfected into 293T cells, and nuclear proteins were prepared 1 day later. Flag-Sox2 proteins and associated proteins were co-immunoprecipitated from nuclear extracts using M2-beads. Immunoprecipitate eluates were used in western blot analyses, and were probed for either α-HDAC2 (top) or α-Flag (bottom). Protein for the control (mock) lane was from un-transfected 293T cells. This experiment was repeated, and similar results were observed.