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(A) Representative FRET traces at 0, 25, 50, 100, and 150 mM of NaCl. (B) The kon and koff at 50 nM of MutL plotted against salt concentration. We observed no significant FRET due to MutL-ssDNA binding above 100 mM NaCl. [NaCl] = 0 mM (n = 130 traces), 25 mM (n = 257 traces), 50 mM (n = 185 traces), and 100 mM (n = 278 traces).
