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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Anal Biochem. 2010 Sep 22;408(2):309–315. doi: 10.1016/j.ab.2010.08.040

Figure 2.

Figure 2

Binding isotherm for the interaction of c-SAHA with HDAC8. The decrease in fluorescence emission intensity at 400 nm (λex = 325 nm) for the titration of 100 nM c-SAHA with increasing concentration of HDAC8 (total) is shown. The solid smooth line is the best fit of the experimental data Qin and Srivastava [29] for the Kd value of 0.16 ± 0.02 μM.