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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Anal Biochem. 2010 Sep 22;408(2):309–315. doi: 10.1016/j.ab.2010.08.040

Figure 3.

Figure 3

Changes in the fluorescence intensity of c-SAHA upon competitive binding SAHA to HDAC8. The decrease in the relative fluorescence emission intensity of a mixture of c-SAHA and SAHA (λex = 325 nm, λem = 400 nm) as a function of total concentration of HDAC8 has been shown. The concentrations of c-SAHA and SAHA were maintained to be 0.5 μM and 2 μM, respectively. The data were analyzed using the software DynaFit [35], and the solid smooth line represents the best fit of the experimental data for the Kd value of HDAC8-SAHA complex as being equal to 0.58 ± 14 μM