. Author manuscript; available in PMC: 2011 Dec 5.

Published in final edited form as: Chem Biol Interact. 2010 Aug 7;188(3):376–385. doi: 10.1016/j.cbi.2010.07.025

Table 1.

Oligonucleotide primers used in site-directed mutagenesis experiments

Residues of GALK	Residues of MVK	Site-directed mutagenesis primers^*

Thr⁷⁷	Leu⁵³	T77L P1 5'-ggtgtctctcctcctcacctctgagggtgccgatg- 3'
Thr⁷⁷	Leu⁵³	T77L P2 5'-caccctcagaggtgaggaggagagacaccagcccatcc-3'

Ser⁷⁹	Asn⁵⁷	S79N P1 5'- ctcctcctcaccaatgagggtgccgatgagccccag -3'
Ser⁷⁹	Asn⁵⁷	S79N P2 5'- Cat cggcaccctcattggtgaggaggagagaca cc -3'

Leu¹⁴⁵	Tyr¹⁴⁹	L145Y P1 5'-ccagctcagcatcctacgaagtggccacgtacaccttcc-3'
		L145Y P2 5'-cgtggccacttcgtaggatgctgagctggacaggcc-3'
		L145A P1 5'-ccagctcagcatccgcggaagtggccacgtacaccttcc-3'
		L145A P2 5'-cgtggccacttccgcggatgctgagctggacaggcc-3'

Trp¹⁰⁶	Thr¹⁰⁴	W106T P1 5'-ggactcctcggacggccaactatgtcaagggagtg-3'
		W106T P2 5'-acatagttggccgtccgaggagtcccaggctccag-3'
		W106A P1 5'-ggactcctcgggcggccaactatgtcaagggagtg-3'
		W106A P2 5'-acatagttggccgcccgaggagtcccaggctccag-3'

Tyr¹⁰⁹	Leu¹⁰⁷	Y109L P1 5'- gtgggccaacttggtcaagggagtgattcagtacta -3'
		Y109L P2 5'- actcccttgaccaagttggcccaccgaggagtcc -3
		Y109A P1 5'- gtgggccaacgctgtcaagggagtgattcagtacta -3
		Y109A P2 5'- actcccttgacagcgttggcccaccgaggagtcc -3

Ser¹⁴⁰	Gly¹⁴⁴	S140G P1 5'-ggggtggcctgggcagctcagcatccttggaagtggc -3'
Ser¹⁴⁰	Gly¹⁴⁴	S140G P2 5'- aggatgctgagctgcccaggccaccccccaggggc -3'

Leu¹³⁵	Pro¹³⁹	L135P P1 5'- ctcagtgcccccggggggtggcctgtccagctcagc -3'
Leu¹³⁵	Pro¹³⁹	L135P P2 5'- ggccaccccccgggggcactgagctgaccaccac -3'

Arg³⁷	Lys⁷	R37K P1 5'- cagcgccgggcaaagtcaacctcatcggggaacacacgg -3'
		R37K P2 5'- atgaggttgactttgcccggcgctgacacggccagctcggg -3'
		R37A P1 5'- cagcgccgggcgccgtcaacctcatcggggaacacacgg -3'
		R37A P2 5'- atgaggttgacggcgcccggcgctgacacggccagctcggg -3'

Loop L1	Loop L1	Loop P1 5'- ggctggtgtctctcctcacccccaacattggtcggctgcagtttccactgcccacagcccag -3'
Loop L1	Loop L1	Loop P2 5'-gcagtggaaactgcagccgaccaatgttgggggtgaggagagacaccagcccatccttgcg-3'