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ESI-MS spectra showing a titration of Chloramphenicol (1–100 µM) into equimolar concentrations of A-site RNA and TO-PRO complex (1 µM). Buffer conditions are 150 mM ammonium acetate, pH 7. Insert shows the relative concentrations free RNA and free chloramphenicol after 40 µM of chloramphenicol was titrated into the pre-bound A-site RNA and TO-PRO complex.
