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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Anal Biochem. 2010 Sep 21;408(2):269–276. doi: 10.1016/j.ab.2010.09.020

Table 1.

FID Assay Results with Paromomycin

dye
(µM)		 RNA
(µM)		 paromomycin
(µM)a 		relative
fluorescence
(%)b
1 0 0 N.D.c
1 1 0 100
1 1 2 87
1 1 10 46
1 1 19 34
1 1 31 28
a

Equimolar concentrations of A-site RNA and TO-PRO (1 µM) were pre-bound in buffer (150 mM ammonium acetate, pH 7) prior to paromomycin addition.

b

Average of three separate experiments.

c

N.D.: not detectable.