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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: Anal Biochem. 2010 Sep 21;408(2):269–276. doi: 10.1016/j.ab.2010.09.020

Table 2.

FID Assay Results with Chloramphenicol

dye
(µM)		 RNA
(µM)		 Chloramphenicol
(µM)a 		relative
fluorescence
(%)b
1 0 0 N.D.c
1 1 0 100
1 1 2 100
1 1 10 100
1 1 19 99
1 1 31 99
a

Equimolar concentrations of A-site RNA and TO-PRO (1 µM) were pre-bound in buffer (150 mM ammonium acetate, pH 7) prior to Chloramphenicol addition.

b

Average of three separate experiments.

c

N.D.: not detectable.